Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.
Anti-IGF2BP2 mouse monoclonal antibody (TA501271) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2 (RC205673).
Immunofluorescent staining of HeLa cells using anti-IGF2BP2 mouse monoclonal antibody (TA501271).
HEK293T cells transfected with either RC205673 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody (TA501271), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2. Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.
Flow cytometric analysis of living Hela cells, using anti-IGF2BP2 antibody (TA501271, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).