HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2. Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.
Anti-IGF2BP2 mouse monoclonal antibody (TA501270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2 (RC205673).
HEK293T cells transfected with either RC205673 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody (TA501270), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-IGF2BP2 antibody (TA501270), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501270, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501270, Dilution 1:50)