Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (RC200610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3. Positive lysates LY400593 (100ug) and LC400593 (20ug) can be purchased separately from OriGene.
Flow cytometric analysis of Jurkat cells, using anti-IFIT3 antibody (TA500726), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Anti-IFIT3 mouse monoclonal antibody (TA500726) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3 (RC200610).
Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody (TA500726).