HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (RC200929, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFI35. Positive lysates LY401696 (100ug) and LC401696 (20ug) can be purchased separately from OriGene.
Anti-IFI35 mouse monoclonal antibody (TA503386) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFI35 (RC200929).
HEK293T cells transfected with either RC200929 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFI35 antibody (TA503386), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-IFI35 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IFI35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503386)