HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3. Positive lysates LY419492 (100ug) and LC419492 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ID3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500811, Dilution 1:50)
Immunofluorescent staining of HeLa cells using anti-ID3 mouse monoclonal antibody (TA500811).
Immunofluorescent staining of HT29 cells using anti-ID3 mouse monoclonal antibody (TA500811).
Anti-ID3 mouse monoclonal antibody (TA500811) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3 (RC200583).