Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-ID3 monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3. Positive lysates LY419492 (100ug) and LC419492 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500791)
Flow cytometric Analysis of Hela cells, using anti-ID3 antibody (TA500791), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ID3 antibody (TA500791), (Red), compared to a nonspecific negative control antibody, (Blue).