HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B. Positive lysates LY405351 (100ug) and LC405351 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
HEK293T cells transfected with either RC209302 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNF144B antibody (TA500722), and then analyzed by flow cytometry.
Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B (RC209302 ).
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody (TA500722), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody (TA500722), (Red), compared to a nonspecific negative control antibody, (Blue).