Hyaluronic Acid Binding Protein
Bind and detect HA in Multiple Applications
Hyaluronan binding protein (HABP) is a ubiquitously present glycoprotein having specific affinity towards hyaluronic acid (HA). HA, also known as Hyaluronan, is a naturally occurring glycosaminoglycan that is functionally important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration. Hyaluronan also exhibits diverse biological functions by interacting with a large number of hyaluronan-binding proteins and cell surface receptors.
Versican G1-domain as HA Binding Protein
AMSBIO’s rHABP was launched as a replacement for the HABP formerly available from Seikagaku. It is produced as a recombinant protein in E. coli BL21(DE3)RIL transfected with human versican G1-domain expression vector pRK172VG1 and is available in purified and biotinylated formats.
rHABP specifically detects Hyaluronic Acid (HA), binding to HA from all species and tissues. It does not cross-react to other Glycosaminoglycans or to DNA.
Recombinant production means that our rHABP has higher safety compared with animal-derived HABP.
See Clark et al (2011) details on expression and comparison with Seikagaku's biotin-HABP from bovine cartilage, carried out by Tony Day and colleagues at Manchester. This paper also cites a number of our antibodies to specific glycosaminoglycans: 10E4 (pan Heparan Sulfate), 1B5, 2B6, 3B3 (Chondroitin Sulfate & Dermatan Sulfate 'stubs’), LY111 (native Chondroitin-4-Sulfate) and 5D4 (pan Keratan Sulfate).
The biotin-HABP product supplied by AMSBIO was vital for us to be able to visualise the tissue distribution of hyaluronan in mouse vs. naked mole-rat skin. The images were so brilliant that we have started using biotin-HABP in other studies, e.g. looking at the effects of colitis on colonic hyaluronan distribution.
The material properties of naked mole-rat hyaluronan.
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Perineuronal net formation during the critical period for neuronal maturation in the hypothalamic arcuate nucleus.
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Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain.
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Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
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AAV vector-mediated secretion of chondroitinase provides a sensitive tracer for axonal arborisations.
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Genome-Wide Association Study of Golden Retrievers Identifies Germ-Line Risk Factors Predisposing to Mast Cell Tumours.
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Molecular composition and expression pattern of the extracellular matrix in a mossy fiber-generating precerebellar nucleus of rat, the prepositus hypoglossi.
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Hyaluronan accumulates with high-fat feeding and contributes to insulin resistance.
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Early Genetic Restoration of Lubricin Expression in Trangenic Mice Mitigates Chondrocyte Peroxynitrite Release and Caspase-3 Activation.
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Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat.
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Molecular weight specific impact of soluble and immobilized hyaluronan on CD44 expressing melanoma cells in 3D collagen matrices.
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