HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1. Positive lysates LY401851 (100ug) and LC401851 (20ug) can be purchased separately from OriGene.
Anti-DNAJB1 mouse monoclonal antibody (TA502244) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1 (RC201762).
Flow cytometric Analysis of Hela cells, using anti-DNAJB1 antibody (TA502244), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and DNAJB1-Knockout 293T cells (KO, Cat# LC811758) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1 monoclonal antibody TA502244, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody.
HEK293T cells transfected with either RC201762 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (TA502244), and then analyzed by flow cytometry.