Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1. Positive lysates LY401851 (100ug) and LC401851 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502195)
HEK293T cells transfected with either RC201762 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (TA502195), and then analyzed by flow cytometry.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and DNAJB1-Knockout 293T cells (KO, Cat# LC811758) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1 monoclonal antibody TA502195, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Anti-DNAJB1 mouse monoclonal antibody (TA502195) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1 (RC201762).