Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody.
HEK293T cells transfected with either RC201762 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (TA502211), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1. Positive lysates LY401851 (100ug) and LC401851 (20ug) can be purchased separately from OriGene.
Anti-DNAJB1 mouse monoclonal antibody (TA502211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1 (RC201762).