HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPB8 (RC201040, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPB8. Positive lysates LY415329 (100ug) and LC415329 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPB8 monoclonal antibody.
Anti-HSPB8 mouse monoclonal antibody (TA501210) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPB8 (RC201040 ).
Flow cytometric Analysis of Jurkat cells, using anti-HSPB8 antibody (TA501210), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC201040 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSPB8 antibody (TA501210), and then analyzed by flow cytometry.