Histones and Nucleosomes
AMSBIO offers both recombinant and purified nucleosomes, as well as full length versions of all the histone proteins. Some biotinylated histones and nucleosomes have been added to our continuously expanding product range.
The major difference between the recombinant proteins made in E.coli and those purified from whole cells is that the recombinant proteins are unmodified. E. coli and other bacteria do not have the enzymes to methylate or acetylate the histone proteins. Nucleosomes purified from mammalian cells will be methylated or acetylated on most histone sites. Our peptides are synthetic, they are methylated on specific sites, making them suitable substrates for our demethylases.