HIF1A Antibody (N-term) (Cat. #TA324790) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of Hela cell stained with HIF1A Antibody (N-term) (Cat#TA324790). Hela cells were incubated with HIF1A primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.