Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ERBB2 monoclonal antibody (1:500).
Anti-ERBB2 mouse monoclonal antibody (TA503526) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2 (RC212583).
HEK293T cells transfected with either RC212583 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERBB2 antibody (TA503526), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2. Positive lysates LY417979 (100ug) and LC417979 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ERBB2-Knockout Hela cells (KO, Cat# LC810070) were separated by SDS-PAGE and immunoblotted with anti-ERBB2 monoclonal antibody TA503526. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:200).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100Â°C for 10min, TA503526)