HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDHD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502834)
HEK293T cells transfected with either RC205967 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDHD2 antibody (TA502834), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-HDHD2 antibody (TA502834), (Red), compared to a nonspecific negative control antibody, (Blue).