HEK293T cells transfected with either RC205967 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDHD2 antibody (TA502847), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-HDHD2 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDHD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502847)