HDAC11 Fluorogenic Assay Kit

The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity|extraction|and chromatography aspects of traditional assays. Using this kit|only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First|the HDAC fluorometric substrate|containing an acetylated lysine side chain|is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay|fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC|the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC|this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity.

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