HDAC11 Fluorogenic Assay Kit

Name: HDAC11 Fluorogenic Assay Kit
Brand: AMS.50687
SKU: AMS.50687
Availability: InStock

Product Code

AMS.50687

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Overview

The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity|extraction|and chromatography aspects of traditional assays. Using this kit|only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First|the HDAC fluorometric substrate|containing an acetylated lysine side chain|is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay|fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC|the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC|this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity.

Product Specifications

More Information
SKU	AMS.50687
Size	96 reactions
Application	Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Gene synonyms	histone deacetylase 11, HDAC-11
Storage	Stable at least one year from date of receipt when stored as directed. Kit components require different storage conditions# Be sure to store each component at the proper temperature upon arrival.
Shipping Temp	-80C (dry ice)
Supplier Name	BPS
Research Areas	Epigenetics

For more details visit our Histone Acetylation page >

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HDAC11 Fluorogenic Assay Kit

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