HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10. Positive lysates LY403142 (100ug) and LC403142 (20ug) can be purchased separately from OriGene.
Immunoprecipitation of HDAC10 by using TrueMab monoclonal anti-HDAC10 antibody (Negative control: IP without adding anti-HDAC10 antibody). For each experiment, 500ul of DDK tagged HDAC10 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-HDAC10 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody (TA500787), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody (TA500787), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).