Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC1 monoclonal antibody.
Anti-HDAC1 mouse monoclonal antibody (TA502142) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1 (RC201745).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC1 (RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1. Positive lysates LY417624 (100ug) and LC417624 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC201745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC1 antibody (TA502142), and then analyzed by flow cytometry.