HDAC Fluorogenic Assay Kit (Green)

The Fluorogenic HDAC Assay Kit is a complete assay systemdesigned to measure histone deacetylase (HDAC) class 1 activity for screeningand profiling applications. The kit comes in a convenient 96-well format|with all thereagents necessary for 100 fluorescent HDAC activity measurements. Inaddition|the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor|Trichostatin A|for use as a positive and negative control. The Fluorogenic HDACAssay Kit is based on a unique fluorogenic substrate and developer combination.This assay method eliminates dealing with the radioactivity|extraction|andchromatography aspects of traditional assays. Using this kit|only two simplesteps on a microtiter plate are needed to analyze the HDAC activity level. First|the HDAC fluorometric substrate|containing an acetylated lysine side chain|isincubated with purified HDAC enzyme. The deacetylation sensitizes thesubstrate so subsequent treatment with the Lysine Developer produces afluorophore that can then be measured using a fluorescence reader at 485 nm(excitation)/528 nm (emission).Our other HDAC kit (#50061) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However|the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate

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