Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HP monoclonal antibody.
Anti-HP mouse monoclonal antibody (TA501699) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HP (RC223612).
HEK293T cells transfected with either RC223612 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HP antibody (TA501699), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (RC223612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP. Positive lysates LY401576 (100ug) and LC401576 (20ug) can be purchased separately from OriGene.