Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HABP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120??C for 3min, TA809299) (1:2000)
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-HABP2 monoclonal antibody (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HABP2 (RC206545, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HABP2 (1:500). Positive lysates LY401333 (100ug) and LC401333 (20ug) can be purchased separately from OriGene.