HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (RC202510, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1. Positive lysates LY400303 (100ug) and LC400303 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GUK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502659)
Flow cytometric Analysis of Hela cells, using anti-GUK1 antibody (TA502659), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-GUK1 antibody (TA502659), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either RC202510 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody (TA502659), and then analyzed by flow cytometry.