HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSPT2 (RC206259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSPT2. Positive lysates LY402649 (100ug) and LC402649 (20ug) can be purchased separately from OriGene.
Immunofluorescent staining of HeLa cells using anti-GSPT2 mouse monoclonal antibody (TA503066).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GSPT2 monoclonal antibody (1:200).
Anti-GSPT2 mouse monoclonal antibody (TA503066) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSPT2 (RC206259).
HEK293T cells transfected with either RC206259 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSPT2 antibody (TA503066), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GSPT2 antibody (TA503066), (Red), compared to a nonspecific negative control antibody, (Blue).