Immunofluorescent staining of HT29 cells using anti-HSPA9 mouse monoclonal antibody (TA500548).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HSPA9 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (RC201397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9. Positive lysates LY401334 (100ug) and LC401334 (20ug) can be purchased separately from OriGene.
Anti-HSPA9 mouse monoclonal antibody (TA500548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).