HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100ug) and LC415912 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA502089), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRHPR monoclonal antibody.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943 ) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA502089 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.