Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943 ) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA501890 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GRHPR mouse monoclonal antibody at 1:150 (TA501890)
HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA501890), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-GRHPR antibody (TA501890), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100ug) and LC415912 (20ug) can be purchased separately from OriGene.