Immunofluorescent staining of HT29 cells using anti-GPI mouse monoclonal antibody (TA501135).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPI (RC201232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI. Positive lysates LY400066 (100ug) and LC400066 (20ug) can be purchased separately from OriGene.
Anti-GPI mouse monoclonal antibody (TA501135) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI (RC201232).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GPI-Knockout 293T cells (KO, Cat#LC840273) were separated by SDS-PAGE and immunoblotted with anti-GPI monoclonal antibody TA501135 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501135, Dilution 1:50)
HEK293T cells transfected with either pCMV6-ENTRY GPI (RC201232) (Red) or empty vector control plasmid (Blue) were immunostained with anti-GPI mouse monoclonal (TA501135), and then analyzed by flow cytometry.