Green Fluorescent Protein (GFP) Polyclonal Antibody
AMSBIO offers purified polyclonal rabbit antibody to Green Fluorescent Protein (GFP) using E. coli-expressed full length GFP as an immunogen. This antibody reacts with wild-type GFP, and its variants, such as EGFP/EBFP.
- Reacts with the wild type GFP from Aequorea victoria and its variants EGFP and EBFP
- Excellent quality due to virtually no background staining; use at 1:5,000-1:10,000 dilution
- Performs well in applications including Western Blotting, Immunofluorescence, Immunoprecipitation
GFP (green fluorescent protein) is a protein of about 27kDa that emits green fluorescent light (emission maximum at 509 nm) when excited by blue or ultraviolet light (excitation maximum at 395 nm). The term GFP usually refers the fluorescent protein originally isolated from the jellyfish Aequorea victoria although many other marine organisms express similar proteins. These green fluorescent proteins from other organisms have different sequences but they resemble the GFP from Aequorea victoria in their molecular structure and properties.
GFP can be fused to a variety of proteins of interest without significantly interfering with their assembly or function and its intrinsic fluorescent properties allow expression as fluorescent protein in virtual any organism. These properties make GFP an ideal fluorescent marker for a broad range of biological applications and have sparked the development of variant fluorescent proteins with different spectral properties to allow simultaneous multi-color reporter experiments.
Primary structure of the Aequorea victoria green-fluorescent protein.
Prasher, DC. et al. (1992) Gene 111(2):229-33
Green fluorescent protein as a marker for gene expression.
Chalfie M, et al. (1994) Science 263(5148):802-5
The green fluorescent protein.
Tsien, RY. (1998) Annu Rev Biochem. 67:509-544