HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN. Positive lysates LY402806 (100ug) and LC402806 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC205986 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GPHN antibody (TA502216), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.
Anti-GPHN mouse monoclonal antibody (TA502216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN (RC205986).