Immunohistochemistry testing of anti-GDNF monoclonal antibody 3C1. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 ug/ml was performed overnight at 4Â°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. GDNF staining by monoclonal antibody 3C1; B. Negative staining without primary antibody.
Immunofluorescence detection of human GDNF expressed in U2OS cells by anti-GDNF monoclonal antibody 3C1. Anti-GDNF antibody 3C1 concentration in IF experiment was 10 ug/ml. Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GDNF specific signal and blue for nuclei. A. GDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).