Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCKR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GCKR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502152)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCKR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502152)
Anti-GCKR mouse monoclonal antibody (TA502152) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR (RC214230).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCKR (RC214230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCKR. Positive lysates LY400576 (100ug) and LC400576 (20ug) can be purchased separately from OriGene.