HEK293T cells transfected with either RC201236 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP1 antibody (TA503589), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from a mouse tissues by using anti-GBP1 monoclonal antibody (1:200).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503589)
Anti-GBP1 mouse monoclonal antibody (TA503589) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP1 (RC201236).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP1 (RC201236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP1. Positive lysates LY400751 (100ug) and LC400751 (20ug) can be purchased separately from OriGene.