Figure from citation: WB, performed with anti-GBE1 antibody (GBE1 mousemonoclonal antibody TA500807, Origene), showed a decrease of protein on skeletal muscle of patients II-5 /II-6, being protein absent on cardiac muscle of a neonateaffected with the congenital variant of glycogenosis IV (GSD-IV or Andersen disease). View Citation
Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody (TA500807), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1. Positive lysates LY400056 (100ug) and LC400056 (20ug) can be purchased separately from OriGene.
Anti-GBE1 mouse monoclonal antibody (TA500807) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1 (RC204152).
Immunoprecipitation (IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.