HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1. Positive lysates LY400290 (100ug) and LC400290 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GAD1 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500330, Dilution 1:50)
Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.