HEK293T cells transfected with either RC204460 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FRK antibody (TA500527), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500527)