HEK293T cells transfected with either RC204275 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FLYWCH2 antibody (TA502774), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLYWCH2 (RC204275, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLYWCH2. Positive lysates LY408693 (100ug) and LC408693 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FLYWCH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502774)
Anti-FLYWCH2 mouse monoclonal antibody (TA502774) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FLYWCH2 (RC204275 ).
Flow cytometric Analysis of Jurkat cells, using anti-FLYWCH2 antibody (TA502774), (Red), compared to a nonspecific negative control antibody, (Blue).