Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-FGFR2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Cross-Reactivity of anti-FGFR2 (TA502797) with FGFR1 and FGFR3, not with FGFR4.HEK293T cells were transfected with the DDK (FLAG)-tagged DNAs of FGFR2 (RC217098), FGFR1 (RC202080), FGFR3 (RC215533), FGFR4 (RC204230) cDNA for 48 hrs and lysed. Equivalent a
Western blot analysis of extracts (35ug) from 2 different cell by using anti-FGFR2 monoclonal antibody (1:250).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2