Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.
HEK293T cells transfected with either RC203506 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody (TA502150), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (RC203506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7. Positive lysates LY419130 (100ug) and LC419130 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and MAPK7-Knockout HeLa cells (KO, Cat# LC830793) were separated by SDS-PAGE and immunoblotted with anti-MAPK7 monoclonal antibody TA502150 (1:200). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Anti-MAPK7 mouse monoclonal antibody (TA502150) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7 (RC203506).