HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1. Positive lysates LY419605 (100ug) and LC419605 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.
Anti-ERCC1 mouse monoclonal antibody (TA501184) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1 (RC200478).
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody (TA501184), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC200478 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC1 antibody (TA501184), and then analyzed by flow cytometry.