HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB3 (RC209954, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB3. Positive lysates LY400725 (100ug) and LC400725 (20ug) can be purchased separately from OriGene.
Western Blot: ErbB 3 Antibody - Western Blot was performed using affinity purified SEQer ERBB3 antibody, aa (146-245) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer ERBB3 antibody, aa (146-245) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown. The detected protein band is estimated at 110 KDa, and the predicated molecular weight of ERBB3 is 140 KDa. The difference of molecular weight between empirical and predicted results are unclear. It could cause by post-translational modification, isotype, proteolytic degradation, gel artifacts or the other reasons.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERBB3 Rabbit Polyclonal antibody. (TA590106)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ERBB3 Rabbit Polyclonal antibody. (TA590106)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ERBB3 Rabbit Polyclonal antibody. (TA590106)