Anti-EFNA2 mouse monoclonal antibody (TA501457) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2 (RC213728).
Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody (TA501457), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (RC213728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2. Positive lysates LY400546 (100ug) and LC400546 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC213728 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EFNA2 antibody (TA501457), and then analyzed by flow cytometry.