HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EpCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EpCAM. Positive lysates LY400847 (100ug) and LC400847 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-EPCAM monoclonal antibody at 1:200.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-TACSTD1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Anti-EpCAM mouse monoclonal antibody (TA500054) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY EpCAM (RC201989)