Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.
HEK293T cells transfected with either RC200368 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EIF2S1 antibody (TA501314), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody (TA501314), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1. Positive lysates LY401321 (100ug) and LC401321 (20ug) can be purchased separately from OriGene.