HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E (1:2000). Positive lysates LY400723 (100ug) and LC400723 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Flow cytometric Analysis of permeabilized A549 cells, using anti-EIF4E antibody (TA503029), (Red), compared to an IgG isotype control, (green) (1:100).
Flow cytometric Analysis of permeabilized Jurkat cells, using anti-EIF4E antibody (TA503029), (Red), compared to an IgG isotype control, (green) (1:100).