HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNA (RC223952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNA. Positive lysates LY409817 (100ug) and LC409817 (20ug) can be purchased separately from OriGene.
Anti-DTNA mouse monoclonal antibody (TA502184) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNA (RC223952).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNA monoclonal antibody.
HEK293T cells transfected with either RC223952 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DTNA antibody (TA502184), and then analyzed by flow cytometry.