Western blot - DLC8 antibody [EP1660Y]; Anti-DYNLL1 antibody [EP1660Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 10 kDa.Observed band size : 10 kDa.
TA303752 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence
Immunohistochemistry (Paraffin-embedded sections) - DLC8 antibody [EP1660Y]; Immunohistochemical staining of paraffin embedded human liver using TA303752 (1/100).
Immunocytochemistry/ Immunofluorescence - DLC8 antibody [EP1660Y]; TA303752 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% mil