HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUPD1 (RC214361, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUPD1. Positive lysates LY424030 (100ug) and LC424030 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Hela cells, using anti-DUPD1 antibody (TA501857), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC214361 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DUPD1 antibody (TA501857), and then analyzed by flow cytometry.