Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP3 (RC219658, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP3. Positive lysates LY417120 (100ug) and LC417120 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC219658 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP3 antibody (TA503291), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-DPP3 antibody (TA503291), (Red), compared to a nonspecific negative control antibody, (Blue).